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Western Blot analysis of KB cells using FSHR Polyclonal Antibody
Western Blot analysis of KB cells using FSHR Polyclonal Antibody
Western Blot analysis of KB cells using FSHR Polyclonal Antibody

FSHR Antibody

CSB-PA002586
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFSHR
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Overview

  • Supplier
    Cusabio
  • Product Name
    FSHR Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2492
  • Target name
    FSHR
  • Target description
    follicle stimulating hormone receptor
  • Target synonyms
    follicle-stimulating hormone receptor; follitropin receptor; FSH receptor; FSHR1; FSHRO; LGR1; ODG1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23945
  • Protein Name
    Follicle-stimulating hormone receptor
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FSH Receptor using anti-FSH Receptor antibody (A00897). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse ovary tissue lysates, Lane 2: HELA whole Cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSH Receptor antigen affinity purified polyclonal antibody (Catalog # A00897) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FSH Receptor at approximately 78KD. The expected band size for FSH Receptor is at 78KD.
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