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Immunohistochemistry analysis in human parathyroid gland and liver tissues using HPA041086 antibody. Corresponding FTO RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human parathyroid gland and liver tissues using HPA041086 antibody. Corresponding FTO RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human parathyroid gland and liver tissues using HPA041086 antibody. Corresponding FTO RNA-seq data are presented for the same tissues.

Anti-FTO Antibody

HPA041086
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFTO
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FTO
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79068
  • Target name
    FTO
  • Target description
    FTO alpha-ketoglutarate dependent dioxygenase
  • Target synonyms
    ALKBH9, BMIQ14, GDFD, IFEX9, alpha-ketoglutarate-dependent dioxygenase FTO, AlkB homolog 9, U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO, U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO, fat mass and obesity associated, fat mass and obesity-associated protein, intragenic FTO exon 9 containing transcript, m6A(m)-demethylase FTO, mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO, mRNA N(6)-methyladenosine demethylase FTO, tRNA N1-methyl adenine demethylase FTO
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9C0B1
  • Protein Name
    Alpha-ketoglutarate-dependent dioxygenase FTO
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of FTO using anti-FTO antibody (A00219-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FTO antigen affinity purified polyclonal antibody (Catalog # A00219-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FTO at approximately 58 kDa. The expected band size for FTO is at 58 kDa.
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