Anti-Alpha-ketoglutarate-dependent dioxygenase FTO [5-2H10]

Absolute Antibody

Anti-Alpha-ketoglutarate-dependent dioxygenase FTO [5-2H10]

9

This antibody is specific for the alpha-ketoglutarate-dependent dioxygenase FTO. This protein is a RNA demethylase that mediates oxidative demethylation of different RNA species, such as mRNAs, tRNAs and snRNAs, and acts as a regulator of fat mass, adipog

This antibody was used to assess the expression of the FTO protein in cell line 3T3-L1. The method used was a western blot (Jiao et al, 2016; pmid:26907332). Immunocytochemistry with this antibody was used for examination of FTO expression in HOC313, MDA-MB-231, SAS, HeLa, 293FT, and MKN45 cells. The experiment was preformed on normal cells and FTO knock-out cells (Hirayama et al, 2020; pmid:32268083).

Western Blot, ImmunoCytoChemistry

Research Use Only

Monoclonal

5-2H10

FTO

FTO alpha-ketoglutarate dependent dioxygenase

AlkB homolog 9; ALKBH9; alpha-ketoglutarate-dependent dioxygenase FTO; BMIQ14; fat mass and obesity associated; fat mass and obesity-associated protein; GDFD; m6A(m)-demethylase FTO; mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; mRNA N(6)-methyladenosine demethylase FTO; tRNA N1-methyl adenine demethylase FTO; U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO

Human

IgG1

Alpha-ketoglutarate-dependent dioxygenase FTO

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Human, Mouse, Rat

-20°C,2°C to 8°C

12352203