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Immunoprecipitation of recombinant human FTO using anti-FTO (human), mAb (FT86-4) (Prod. No. AG-20A-0064). Recombinant human FTO proteins at different concentrations were precipitated by FT86-4. The precipitated proteins were separated by SDS-PAGE, electr
Immunoprecipitation of recombinant human FTO using anti-FTO (human), mAb (FT86-4) (Prod. No. AG-20A-0064). Recombinant human FTO proteins at different concentrations were precipitated by FT86-4. The precipitated proteins were separated by SDS-PAGE, electr
Immunoprecipitation of recombinant human FTO using anti-FTO (human), mAb (FT86-4) (Prod. No. AG-20A-0064). Recombinant human FTO proteins at different concentrations were precipitated by FT86-4. The precipitated proteins were separated by SDS-PAGE, electr

anti-FTO, mAb (FT86-4)

AG-20A-0064
AdipoGen Life Sciences
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetFTO
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Overview

  • Supplier
    AdipoGen Life Sciences
  • Product Name
    anti-FTO, mAb (FT86-4)
  • Delivery Days Customer
    10
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    FT86-4
  • Concentration
    1 mg/ml
  • Gene ID79068
  • Target name
    FTO
  • Target description
    FTO alpha-ketoglutarate dependent dioxygenase
  • Target synonyms
    AlkB homolog 9; ALKBH9; alpha-ketoglutarate-dependent dioxygenase FTO; BMIQ14; fat mass and obesity associated; fat mass and obesity-associated protein; GDFD; m6A(m)-demethylase FTO; mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; mRNA N(6)-methyladenosine demethylase FTO; tRNA N1-methyl adenine demethylase FTO; U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9C0B1
  • Protein Name
    Alpha-ketoglutarate-dependent dioxygenase FTO
  • Scientific Description
    FTO (Fat mass-and obesity-associated gene) is the responsible gene for mouse fused toes mutation. An association between FTO genotype and type 2 diabetes has been confirmed. The presence of the FTO rs9939609 A-allele was found to be positively correlated with other symptoms of the metabolic syndrome, including higher fasting insulin, glucose, triglycerides, and lower HDL-cholesterol. - Monoclonal Antibody. Recognizes human, mouse and rat FTO. Isotype: Mouse IgG1kappa. Clone: FT86-4. Applications: ELISA, IP, WB. Liquid. 0.2microm-filtered solution in PBS, pH 7.4. Contains no preservatives. FTO (Fat mass-and obesity-associated gene) is the responsible gene for mouse fused toes mutation. An association between FTO genotype and type 2 diabetes has been confirmed. The presence of the FTO rs9939609 A-allele was found to be positively correlated with other symptoms of the metabolic syndrome, including higher fasting insulin, glucose, triglycerides, and lower HDL-cholesterol.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FTO using anti-FTO antibody (A00219-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FTO antigen affinity purified polyclonal antibody (Catalog # A00219-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FTO at approximately 58 kDa. The expected band size for FTO is at 58 kDa.
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