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Immunohistochemical staining of human epididymis shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human epididymis shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human epididymis shows strong cytoplasmic positivity in glandular cells.

Anti-FURIN Antibody

HPA067869
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetFURIN
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FURIN
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5045
  • Target name
    FURIN
  • Target description
    furin, paired basic amino acid cleaving enzyme
  • Target synonyms
    dibasic processing enzyme; FES upstream region; FUR; furin; furin, membrane associated receptor protein; PACE; paired basic amino acid residue-cleaving enzyme; PCSK3; proprotein convertase subtilisin/kexin 3; proprotein convertase subtilisin/kexin type 3; SPC1; subtilisin-like proprotein convertase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09958
  • Protein Name
    Furin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Lane 1: HepG2 Cells; Lane 2: Hela Cells; Lane 3: Hela Cells; Lane 4: MCF-7 Cells; Probed with Furin (8C3) Monoclonal Antibody (bsm-54283R) at 1:500 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA009068LA01HU at dilution of 1:100
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Figure 1. Western blot analysis of Furin using anti-Furin antibody (PB9182). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human Furin protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Furin antigen affinity purified polyclonal antibody (Catalog # PB9182) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Furin at approximately 40 kDa. The expected band size for Furin is at 40 kDa.
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