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Anti-GABRG2 (Center) Antibody

102-20422
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGABRG2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-GABRG2 (Center) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB23939
  • Gene ID2566
  • Target name
    GABRG2
  • Target description
    gamma-aminobutyric acid type A receptor subunit gamma2
  • Target synonyms
    CAE2; DEE74; ECA2; EIEE74; FEB8; GABA(A) receptor subunit gamma-2; GABA(A) receptor, gamma 2; gamma-aminobutyric acid (GABA) A receptor, gamma 2; gamma-aminobutyric acid receptor subunit gamma-2; gamma-aminobutyric acid type A receptor gamma2 subunit; GEFSP3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18507
  • Protein Name
    Gamma-aminobutyric acid receptor subunit gamma-2
  • Scientific Description
    Rabbit Anti-GABRG2 (Center) Antibody, 400 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GABA A Receptor gamma 2/GABRG2 using anti-GABA A Receptor gamma 2/GABRG2 antibody (A02361-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GABA A Receptor gamma 2/GABRG2 antigen affinity purified polyclonal antibody (Catalog # A02361-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GABA A Receptor gamma 2/GABRG2 at approximately 50KD. The expected band size for GABA A Receptor gamma 2/GABRG2 is at 50KD.
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