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Immunofluorescence staining of Hela cells with CSB-PA009152LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of Hela cells with CSB-PA009152LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of Hela cells with CSB-PA009152LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

GABRG2 Antibody

CSB-PA009152LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
ReactivityHuman
TargetGABRG2
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Overview

  • Supplier
    Cusabio
  • Product Name
    GABRG2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2566
  • Target name
    GABRG2
  • Target description
    gamma-aminobutyric acid type A receptor subunit gamma2
  • Target synonyms
    CAE2, DEE74, ECA2, EIEE74, FEB8, GEFSP3, gamma-aminobutyric acid receptor subunit gamma-2, GABA(A) receptor subunit gamma-2, GABA(A) receptor, gamma 2, GABAAR subunit gamma-2, gamma-aminobutyric acid (GABA) A receptor, gamma 2, gamma-aminobutyric acid type A receptor gamma2 subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18507
  • Protein Name
    Gamma-aminobutyric acid receptor subunit gamma-2
  • Scientific Description
    Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GABA A Receptor gamma 2/GABRG2 using anti-GABA A Receptor gamma 2/GABRG2 antibody (A02361-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GABA A Receptor gamma 2/GABRG2 antigen affinity purified polyclonal antibody (Catalog # A02361-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GABA A Receptor gamma 2/GABRG2 at approximately 50KD. The expected band size for GABA A Receptor gamma 2/GABRG2 is at 50KD.
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