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Figure 1. Western blotting validation for Anti-GADD 4 gamma GADD45G Antibody A04681 Western Blot (WB) analysis of specific cells using GADD 45gamma polyclonal antibody. Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Figure 1. Western blotting validation for Anti-GADD 4 gamma GADD45G Antibody A04681 Western Blot (WB) analysis of specific cells using GADD 45gamma polyclonal antibody. Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Figure 1. Western blotting validation for Anti-GADD 4 gamma GADD45G Antibody A04681 Western Blot (WB) analysis of specific cells using GADD 45gamma polyclonal antibody. Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Anti-GADD 4 gamma GADD45G Antibody

A04681
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGADD45G
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-GADD 4 gamma GADD45G Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID10912
  • Target name
    GADD45G
  • Target description
    growth arrest and DNA damage inducible gamma
  • Target synonyms
    CR6, DDIT2, GADD45gamma, GRP17, growth arrest and DNA damage-inducible protein GADD45 gamma, DDIT-2, DNA damage-inducible transcript 2 protein, GADD45-gamma, cytokine-responsive protein CR6, gadd-related protein, 17 kD
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95257
  • Protein Name
    Growth arrest and DNA damage-inducible protein GADD45 gamma
  • Scientific Description
    Boster Bio Anti-GADD 4 gamma GADD45G Antibody catalog # A04681. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of GADD45G using anti-GADD45G antibody (A04681-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat testis tissue lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse skeletal muscle tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GADD45G antigen affinity purified polyclonal antibody (Catalog # A04681-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GADD45G at approximately 19KD. The expected band size for GADD45G is at 17KD.
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