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Immunohistochemical staining of human pituitary gland shows moderate to strong cytoplasmic positivity in endocrine glandular cells.
Immunohistochemical staining of human pituitary gland shows moderate to strong cytoplasmic positivity in endocrine glandular cells.
Immunohistochemical staining of human pituitary gland shows moderate to strong cytoplasmic positivity in endocrine glandular cells.

Anti-GAL Antibody

HPA049864
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGAL
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GAL
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51083
  • Target name
    GAL
  • Target description
    galanin and GMAP prepropeptide
  • Target synonyms
    ETL8, GAL-GMAP, GALN, GLNN, GMAP, galanin peptides, galanin prepropeptide, galanin-message-associated peptide, galanin-related peptide, galanin/GMAP prepropeptide
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22466
  • Protein Name
    Galanin peptides
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of Galanin using anti-Galanin antibody (A00606-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat small intestine tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse small intestine tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galanin antigen affinity purified polyclonal antibody (Catalog # A00606-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galanin at approximately 19KD. The expected band size for Galanin is at 13KD.
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