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Anti-Galanin Antibody

130-10452
RayBiotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetGAL
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-Galanin Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Concentration
    1 mg/ml
  • Gene ID51083
  • Target name
    GAL
  • Target description
    galanin and GMAP prepropeptide
  • Target synonyms
    ETL8, GAL-GMAP, GALN, GLNN, GMAP, galanin peptides, galanin prepropeptide, galanin-message-associated peptide, galanin-related peptide, galanin/GMAP prepropeptide
  • Host
    Rabbit
  • Protein IDP22466
  • Protein Name
    Galanin peptides
  • Scientific Description
    Rabbit anti-Galanin Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Galanin using anti-Galanin antibody (A00606-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat small intestine tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse small intestine tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galanin antigen affinity purified polyclonal antibody (Catalog # A00606-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Galanin at approximately 19KD. The expected band size for Galanin is at 13KD.
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