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Anti-GALP Antibody

130-10416
RayBiotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetGALP
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-GALP Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Concentration
    1 mg/ml
  • Gene ID85569
  • Target name
    GALP
  • Target description
    galanin like peptide
  • Target synonyms
    GAL, galanin-like peptide, alarin, gal-like peptide
  • Host
    Rabbit
  • Protein IDQ9UBC7
  • Protein Name
    Galanin-like peptide
  • Scientific Description
    Rabbit anti-GALP Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GALP using anti-GALP antibody (A04786-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GALP antigen affinity purified polyclonal antibody (Catalog # A04786-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GALP at approximately 15,18 kDa. The expected band size for GALP is at 13 kDa.
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