Immunocytochemistry of HeLa cells using gamma H2AX Rabbit mAb RM224 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Immunocytochemistry of HeLa cells using gamma H2AX Rabbit mAb RM224 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
anti-Gamma H2AX (phospho-Ser139), Rabbit Monoclonal (RM224)
REV-31-1104-00
ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry, Other Application
Product group Antibodies
ReactivityVertebrate
TargetH2AX
Overview
- SupplierRevMAb Biosciences
- Product Nameanti-Gamma H2AX (phospho-Ser139), Rabbit Monoclonal (RM224)
- Delivery Days Customer5
- Antibody SpecificityThis antibody reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones.
- ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry, Other Application
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRM224
- Concentration1 mg/ml
- FormulationLiquid
- Gene ID3014
- Target nameH2AX
- Target descriptionH2A.X variant histone
- Target synonymsH2A histone family member X; H2A.X; H2A/X; H2AFX; H2AX histone; histone H2A.x; histone H2AX
- HostRabbit
- IsotypeIgG
- Protein IDP16104
- Protein NameHistone H2AX
- Scientific DescriptionHistones are proteins that package DNA into nucleosomes. Histones are responsible for maintaining the shape and structure of a nucleosome. One chromatin molecule is composed of at least one of each core histones per 100 base pairs of DNA. There are five families of histones known to date; these histones are termed H1/H5, H2A, H2B, H3, and H4. H2A is considered a core histone, along with H2B, H3 and H4. Core formation first occurs through the interaction of two H2A molecules. Then, H2A forms a dimer with H2B; the core molecule is complete when H3-H4 also attaches to form a tetramer. Histone H2A is composed of non-allelic variants, including H2A.1, H2A.2, H2A.X, and H2A.Z. H2A packages DNA molecules into chromatin and has been correlated with DNA modification and epigenetics. H2A plays a major role in determining the overall structure of chromatin and regulates gene expression. Protein modification on histone H2A exist and can sometimes result in a change in function. Different H2A variants were exploited to have different functions, genetic sequences and modifications. - Recombinant Antibody. This antibody reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones. Applications: WB, ELISA, Multiplex, ICC. Source: Rabbit. Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. Histones are proteins that package DNA into nucleosomes. Histones are responsible for maintaining the shape and structure of a nucleosome. One chromatin molecule is composed of at least one of each core histones per 100 base pairs of DNA. There are five families of histones known to date; these histones are termed H1/H5, H2A, H2B, H3, and H4. H2A is considered a core histone, along with H2B, H3 and H4. Core formation first occurs through the interaction of two H2A molecules. Then, H2A forms a dimer with H2B; the core molecule is complete when H3-H4 also attaches to form a tetramer. Histone H2A is composed of non-allelic variants, including H2A.1, H2A.2, H2A.X, and H2A.Z. H2A packages DNA molecules into chromatin and has been correlated with DNA modification and epigenetics. H2A plays a major role in determining the overall structure of chromatin and regulates gene expression. Protein modification on histone H2A exist and can sometimes result in a change in function. Different H2A variants were exploited to have different functions, genetic sequences and modifications.
- ReactivityVertebrate
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203