
Histone H2A.X antibody detects Histone H2A.X protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Histone H2A.X stained by Histone H2A.X antibody (GTX108272) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Histone H2A.X antibody
GTX108272
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetH2AX
Overview
- SupplierGeneTex
- Product NameHistone H2A.X antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.19 mg/ml
- ConjugateUnconjugated
- Gene ID3014
- Target nameH2AX
- Target descriptionH2A.X variant histone
- Target synonymsH2A.X, H2A/X, H2AFX, histone H2AX, H2A histone family member X, H2AX histone, histone H2A.x
- HostRabbit
- IsotypeIgG
- Protein IDP16104
- Protein NameHistone H2AX
- Scientific DescriptionHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![Histone H2A.X antibody immunoprecipitates H2AFX protein in IP experiments. IP samples: Jurkat whole cell extract A. 40 μg Jurkat whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of H2AFX protein by 4 μg Histone H2A.X antibody (GTX108272) 15 % SDS-PAGE The immunoprecipitated H2AFX protein was detected by Histone H2A.X antibody (GTX108272) diluted at 1:2000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent] Histone H2A.X antibody immunoprecipitates H2AFX protein in IP experiments. IP samples: Jurkat whole cell extract A. 40 μg Jurkat whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of H2AFX protein by 4 μg Histone H2A.X antibody (GTX108272) 15 % SDS-PAGE The immunoprecipitated H2AFX protein was detected by Histone H2A.X antibody (GTX108272) diluted at 1:2000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX108272/GTX108272_39778_IP_w_23060120_531.webp)















![Histone H2A.XS139ph (phospho Ser139) antibody [HL1299] detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Histone H2A.XS139ph (phospho Ser139) stained by Histone H2A.XS139ph (phospho Ser139) antibody [HL1299] (GTX636713) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636713/GTX636713_44620_20220408_IHC-P_M_22071401_185.webp)
![Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.X antibody [HL3515] (GTX641412) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX641412/GTX641412_T-45614_20241206_WB_R_24121100_257.webp)