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Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Anti-GATB Antibody

HPA042610
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetGATB
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GATB
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5188
  • Target name
    GATB
  • Target description
    glutamyl-tRNA amidotransferase subunit B
  • Target synonyms
    COXPD41; cytochrome c oxidase assembly factor PET112 homolog; cytochrome oxidase assembly factor PET112 homolog; glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial; glutamyl-tRNA(Gln) amidotransferase, subunit B; HSPC199; PET112; PET112 homolog; PET112L
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75879
  • Protein Name
    Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PET112/GATB using anti-PET112/GATB antibody (A08058-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PET112/GATB antigen affinity purified polyclonal antibody (Catalog # A08058-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PET112/GATB at approximately 62 kDa. The expected band size for PET112/GATB is at 62 kDa.
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Western Blot Positive WB detected in: K562 whole cell lysate, HepG2 whole cell lysate All lanes: GATB antibody at 3.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 62 kDa Observed band size: 62 kDa
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