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Western Blot Positive WB detected in: K562 whole cell lysate, HepG2 whole cell lysate All lanes: GATB antibody at 3.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 62 kDa Observed band size: 62 kDa
Western Blot Positive WB detected in: K562 whole cell lysate, HepG2 whole cell lysate All lanes: GATB antibody at 3.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 62 kDa Observed band size: 62 kDa
Western Blot Positive WB detected in: K562 whole cell lysate, HepG2 whole cell lysate All lanes: GATB antibody at 3.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 62 kDa Observed band size: 62 kDa

GATB Antibody

CSB-PA017792LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetGATB
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Overview

  • Supplier
    Cusabio
  • Product Name
    GATB Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5188
  • Target name
    GATB
  • Target description
    glutamyl-tRNA amidotransferase subunit B
  • Target synonyms
    COXPD41; cytochrome c oxidase assembly factor PET112 homolog; cytochrome oxidase assembly factor PET112 homolog; glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial; glutamyl-tRNA(Gln) amidotransferase, subunit B; HSPC199; PET112; PET112 homolog; PET112L
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75879
  • Protein Name
    Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial
  • Scientific Description
    Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PET112/GATB using anti-PET112/GATB antibody (A08058-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PET112/GATB antigen affinity purified polyclonal antibody (Catalog # A08058-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PET112/GATB at approximately 62 kDa. The expected band size for PET112/GATB is at 62 kDa.
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