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Western blot analysis of GFR alpha 1 in crude membrane fractions of human brain (B)
Western blot analysis of GFR alpha 1 in crude membrane fractions of human brain (B)
Western blot analysis of GFR alpha 1 in crude membrane fractions of human brain (B)

Anti-GFR alpha 1 GFRA1 Antibody

A02897
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGFRA1
0.1 mg
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-GFR alpha 1 GFRA1 Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID2674
  • Target name
    GFRA1
  • Target description
    GDNF family receptor alpha 1
  • Target synonyms
    GDNFR, GDNFR-alpha-1, GDNFRA, GFR-ALPHA-1, GFRalpha-1, RET1L, RETL1, RHDA4, TRNR1, GDNF family receptor alpha-1, GDNF receptor alpha 1d, GDNF receptor alpha 1e, GPI-linked anchor protein, Glial cell line-derived neurotrophic factor receptor alpha, PI-linked cell-surface accessory protein, RET ligand 1, TGF-beta-related neurotrophic factor receptor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP56159
  • Protein Name
    GDNF family receptor alpha-1
  • Scientific Description
    Boster Bio Anti-GFR alpha 1 GFRA1 Antibody (Catalog # A02897). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GFRA1 using anti-GFRA1 antibody (PB9202). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human GFRA1 protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GFRA1 antigen affinity purified polyclonal antibody (Catalog # PB9202) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GFRA1 at approximately 39 kDa. The expected band size for GFRA1 is at 39 kDa.
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