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Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.

Anti-GGA2 Antibody

HPA043313
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetGGA2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GGA2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23062
  • Target name
    GGA2
  • Target description
    golgi associated, gamma adaptin ear containing, ARF binding protein 2
  • Target synonyms
    ADP-ribosylation factor-binding protein GGA2; gamma-adaptin-related protein 2; golgi-localized, gamma ear-containing, ARF-binding protein 2; VEAR; VHS domain and ear domain of gamma-adaptin; VHS domain and ear domain-containing protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UJY4
  • Protein Name
    ADP-ribosylation factor-binding protein GGA2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western blot All lanes: ADP-ribosylation factor-binding protein GGA2 antibody at 10ug/ml Lane 1: Rat brain tissue Lane 2: Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 67 kDa Observed band size: 67 kDa
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Figure 1. Western blot analysis of GGA2 using anti-GGA2 antibody (A04016-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GGA2 antigen affinity purified polyclonal antibody (Catalog # A04016-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GGA2 at approximately 70 kDa. The expected band size for GGA2 is at 67 kDa.
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