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Western blot All lanes: ADP-ribosylation factor-binding protein GGA2 antibody at 10ug/ml Lane 1: Rat brain tissue Lane 2: Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 67 kDa Observed band size: 67 kDa
Western blot All lanes: ADP-ribosylation factor-binding protein GGA2 antibody at 10ug/ml Lane 1: Rat brain tissue Lane 2: Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 67 kDa Observed band size: 67 kDa
Western blot All lanes: ADP-ribosylation factor-binding protein GGA2 antibody at 10ug/ml Lane 1: Rat brain tissue Lane 2: Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 67 kDa Observed band size: 67 kDa

GGA2 Antibody

CSB-PA891942ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGGA2
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Overview

  • Supplier
    Cusabio
  • Product Name
    GGA2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23062
  • Target name
    GGA2
  • Target description
    golgi associated, gamma adaptin ear containing, ARF binding protein 2
  • Target synonyms
    VEAR, ADP-ribosylation factor-binding protein GGA2, VHS domain and ear domain of gamma-adaptin, VHS domain and ear domain-containing protein, gamma-adaptin-related protein 2, golgi-localized, gamma ear-containing, ARF-binding protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UJY4
  • Protein Name
    ADP-ribosylation factor-binding protein GGA2
  • Scientific Description
    Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GGA2 using anti-GGA2 antibody (A04016-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GGA2 antigen affinity purified polyclonal antibody (Catalog # A04016-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GGA2 at approximately 70 kDa. The expected band size for GGA2 is at 67 kDa.
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