Anti-gHgL [CL40]

Absolute Antibody

Anti-gHgL [CL40]

9

The antibody binds to an epitope in gH D-II that overlaps substantially with a region observed to interact with the gp42 HP. In particular the antibody interacts with gH D-II using light chain contacts composed of L-CDR3 with the N-terminal end of gH:2alp

The antibody blocks EBV-mediated membrane fusion with both B cells and epithelial cells and it retains its inhibitory activity as a monomeric Fab. Flow cytometry cell counts showed that Fab fragment inhibits virus entry. Neutralization experiments with epithelial cells using an EBV-GFP reporter virus and purified Fab were performed. Fluorescence images obtained at 24 h postinfection and flow cytometry cell counts showed that the Fab inhibits virus entry. Fab inhibition of cell-cell fusion and EBV infection exhibited similar concentration dependence. (PMID: 28939750) The cell-surface expression of gHgL was evaluated using the antibody by CELISA. (PMID: 27929061)

Flow Cytometry, Western Blot, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

CL40

BKRF2

envelope glycoprotein L

envelope glycoprotein L; HHV4_BKRF2

Human

IgG1

Envelope glycoprotein L

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Virus

-20°C,2°C to 8°C

12352203