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Immunohistochemical staining of human colon shows strong nuclear positivity combined with distinct granular cytoplasmic staining in glandular cells.
Immunohistochemical staining of human colon shows strong nuclear positivity combined with distinct granular cytoplasmic staining in glandular cells.
Immunohistochemical staining of human colon shows strong nuclear positivity combined with distinct granular cytoplasmic staining in glandular cells.

Anti-GINS4 Antibody

HPA026580
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGINS4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GINS4
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84296
  • Target name
    GINS4
  • Target description
    GINS complex subunit 4
  • Target synonyms
    SLD5, DNA replication complex GINS protein SLD5, SLD5 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BRT9
  • Protein Name
    DNA replication complex GINS protein SLD5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SLD5/GINS4 using anti-SLD5/GINS4 antibody (A10548-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLD5/GINS4 antigen affinity purified polyclonal antibody (Catalog # A10548-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLD5/GINS4 at approximately 28 kDa. The expected band size for SLD5/GINS4 is at 26 kDa.
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