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Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.

Anti-GIPC1 Antibody

HPA043958
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetGIPC1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GIPC1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10755
  • Target name
    GIPC1
  • Target description
    GIPC PDZ domain containing family member 1
  • Target synonyms
    C19orf3; GAIP C-terminus-interacting protein; GIPC; GLUT1 C-terminal binding protein; GLUT1CBP; Hs.6454; IGF-1 receptor interacting protein 1; IIP-1; NIP; OPDM2; PDZ domain-containing protein GIPC1; regulator of G-protein signalling 19 interacting protein 1; RGS19-interacting protein 1; RGS19IP1; RGS-GAIP-interacting protein; SEMCAP; SYNECTIIN; SYNECTIN; tax interaction protein 2; TIP-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14908
  • Protein Name
    PDZ domain-containing protein GIPC1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western blot analysis using Anti-GIPC1 antibody HPA061786 (A) shows similar pattern to independent antibody HPA043958 (B).
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Figure 1. Western blot analysis of GIPC1 using anti-GIPC1 antibody (A04969-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat skeletal muscle tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: rat NRK whole cell lysates, Lane 6: mouse skeletal muscle tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GIPC1 antigen affinity purified polyclonal antibody (Catalog # A04969-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GIPC1 at approximately 40 kDa. The expected band size for GIPC1 is at 36 kDa.
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