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GIPC1 / GIPC Antibody

LS-C830106
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetGIPC1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GIPC1 / GIPC Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:5000 - 1:10000), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10755
  • Target name
    GIPC1
  • Target description
    GIPC PDZ domain containing family member 1
  • Target synonyms
    C19orf3; GAIP C-terminus-interacting protein; GIPC; GLUT1 C-terminal binding protein; GLUT1CBP; Hs.6454; IGF-1 receptor interacting protein 1; IIP-1; NIP; OPDM2; PDZ domain-containing protein GIPC1; regulator of G-protein signalling 19 interacting protein 1; RGS19-interacting protein 1; RGS19IP1; RGS-GAIP-interacting protein; SEMCAP; SYNECTIIN; SYNECTIN; tax interaction protein 2; TIP-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GIPC1 using anti-GIPC1 antibody (A04969-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat skeletal muscle tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: rat NRK whole cell lysates, Lane 6: mouse skeletal muscle tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GIPC1 antigen affinity purified polyclonal antibody (Catalog # A04969-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GIPC1 at approximately 40 kDa. The expected band size for GIPC1 is at 36 kDa.
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