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Anti-GIT2 [N83/48R]

Ab02309-10.0
Absolute Antibody
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetGIT2
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-GIT2 [N83/48R]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody is recommended for detection and analysis of GIT2 by immunohistochemistry, immunoprecipitation and western blot. For instance, the mouse version of this antibody was used to detect GIT2 by western blot in : extracts of rat brain membranes (RBM) and COS cells transiently transfected with Flag-tagged GIT1 and GIT2 and untagged Kv2.1 plasmids. Furthermore it was used to detect GIT2 by immunohistochemistry in adult rat hippocampus.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC; IP; WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    N83/48R
  • Gene ID9815
  • Target name
    GIT2
  • Target description
    GIT ArfGAP 2
  • Target synonyms
    ARF GAP GIT2; ARF GTPase-activating protein GIT2; CAT2; CAT-2; cool-associated, tyrosine phosphorylated protein 2; cool-interacting tyrosine-phosphorylated protein 2; G protein-coupled receptor kinase interacting ArfGAP 2; G protein-coupled receptor kinase-interactor 2; GRK-interacting protein 2; Paxillin kinase linker; PKL
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDQ14161
  • Protein Name
    ARF GTPase-activating protein GIT2
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human, mouse, rat
  • Reactivity Supplier Note
    This antibody was raised against a fusion protein of amino acids 450-759 of human GIT2.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GIT2 using anti-GIT2 antibody (A04441-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GIT2 antigen affinity purified polyclonal antibody (Catalog # A04441-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GIT2 at approximately 85 kDa. The expected band size for GIT2 is at 85 kDa.
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