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Western Blot analysis of HepG2 cells using GIT2 Polyclonal Antibody
Western Blot analysis of HepG2 cells using GIT2 Polyclonal Antibody
Western Blot analysis of HepG2 cells using GIT2 Polyclonal Antibody

GIT2 Antibody

CSB-PA002690
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetGIT2
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Overview

  • Supplier
    Cusabio
  • Product Name
    GIT2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9815
  • Target name
    GIT2
  • Target description
    GIT ArfGAP 2
  • Target synonyms
    ARF GAP GIT2; ARF GTPase-activating protein GIT2; CAT2; CAT-2; cool-associated, tyrosine phosphorylated protein 2; cool-interacting tyrosine-phosphorylated protein 2; G protein-coupled receptor kinase interacting ArfGAP 2; G protein-coupled receptor kinase-interactor 2; GRK-interacting protein 2; Paxillin kinase linker; PKL
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14161
  • Protein Name
    ARF GTPase-activating protein GIT2
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GIT2 using anti-GIT2 antibody (A04441-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GIT2 antigen affinity purified polyclonal antibody (Catalog # A04441-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GIT2 at approximately 85 kDa. The expected band size for GIT2 is at 85 kDa.
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