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Anti-GITR [T10P2H9B9]

Ab00727-1.1
Absolute Antibody
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTNFRSF18
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-GITR [T10P2H9B9]
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA; IHC; WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    T10P2H9*B9
  • Gene ID8784
  • Target name
    TNFRSF18
  • Target description
    TNF receptor superfamily member 18
  • Target synonyms
    AITR, CD357, ENERGEN, GITR, GITR-D, tumor necrosis factor receptor superfamily member 18, TNF receptor superfamily activation-inducible protein, activation-inducible TNFR family receptor, glucocorticoid-induced TNFR-related protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9Y5U5
  • Protein Name
    Tumor necrosis factor receptor superfamily member 18
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TNFRSF18 using anti-TNFRSF18 antibody (A03125-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysate, Lane 2: mouse kidney tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFRSF18 antigen affinity purified polyclonal antibody (Catalog # A03125-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNFRSF18 at approximately 28KD. The expected band size for TNFRSF18 is at 26KD.
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