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Immunohistochemical staining of human eye shows strong positivity in the lens.
Immunohistochemical staining of human eye shows strong positivity in the lens.
Immunohistochemical staining of human eye shows strong positivity in the lens.

Anti-GJA3 Antibody

HPA014821
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGJA3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GJA3 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2700
  • Target name
    GJA3
  • Target description
    gap junction protein alpha 3
  • Target synonyms
    CTRCT14, CX46, CZP3, gap junction alpha-3 protein, connexin-46, gap junction alpha 3, gap junction protein, alpha 3, 46kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y6H8
  • Protein Name
    Gap junction alpha-3 protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of GJA3 using anti-GJA3 antibody (PB9596). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug, Lane 2: Rat Kidney Tissue Lysate at 50ug, Lane 3: Human Placenta Tissue Lysate at 50ug, Lane 4: NIH3T3 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GJA3 antigen affinity purified polyclonal antibody (Catalog # PB9596) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GJA3 at approximately 55 kDa. The expected band size for GJA3 is at 47 kDa.
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