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GJA3 Antibody

CSB-PA008832
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGJA3
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Overview

  • Supplier
    Cusabio
  • Product Name
    GJA3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2700
  • Target name
    GJA3
  • Target description
    gap junction protein alpha 3
  • Target synonyms
    CTRCT14, CX46, CZP3, gap junction alpha-3 protein, connexin-46, gap junction alpha 3, gap junction protein, alpha 3, 46kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y6H8
  • Protein Name
    Gap junction alpha-3 protein
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GJA3 using anti-GJA3 antibody (PB9596). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug, Lane 2: Rat Kidney Tissue Lysate at 50ug, Lane 3: Human Placenta Tissue Lysate at 50ug, Lane 4: NIH3T3 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GJA3 antigen affinity purified polyclonal antibody (Catalog # PB9596) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GJA3 at approximately 55 kDa. The expected band size for GJA3 is at 47 kDa.
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