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Anti-Glypican 3 [15G1]

Ab03202-23.0
Absolute Antibody
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Rat
TargetGPC3
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Glypican 3 [15G1]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody can bind human glypican 3 expressed on the surface of 293S cells, HepG2 X1 cells and JHH7 cells in a flow cytometric assay. This antibody reacts with human rat and cynomolgus monkey GPC3 in a flow cytometric assay. The binding characterization of this antibody to huGPC3, cynoGPC3, and HepG2 cells was done using ELISA and immunohistochemistry. (US9926377).
  • Applications
    Flow Cytometry, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    FC; ELISA; IHC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    15G1
  • Gene ID2719
  • Target name
    GPC3
  • Target description
    glypican 3
  • Target synonyms
    DGSX, GTR2-2, MXR7, OCI-5, SDYS, SGB, SGBS, SGBS1, glypican-3, glypican proteoglycan 3, heparan sulphate proteoglycan, intestinal protein OCI-5, secreted glypican-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51654
  • Protein Name
    Glypican-3
  • Scientific Description
    This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
  • Reactivity
    Human, Monkey, Rat
  • Reactivity Supplier
    Human, cynomolgus monkey, rat
  • Reactivity Supplier Note
    The original antibody was generated by immunizing BALB/c mice with a recombinant human GPC3 extracellular domain (ECD, amino acids of 1-547) fused to a C-terminal Flag (RADYKDDDDK).
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Glypican 3/GPC3 using anti-Glypican 3/GPC3 antibody (A01922-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glypican 3/GPC3 antigen affinity purified polyclonal antibody (Catalog # A01922-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glypican 3/GPC3 at approximately 66 kDa. The expected band size for Glypican 3/GPC3 is at 66 kDa.
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