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Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.

Anti-GNAI2 Antibody

HPA007704
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGNAI2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GNAI2
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2771
  • Target name
    GNAI2
  • Target description
    G protein subunit alpha i2
  • Target synonyms
    GIP, GNAI2B, HG1C, H_LUCA15.1, H_LUCA16.1, guanine nucleotide-binding protein G(i) subunit alpha-2, GTP-binding regulatory protein Gi alpha-2 chain, adenylate cyclase-inhibiting G alpha protein, guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2, guanine nucleotide-binding protein G(i), alpha-2 subunit, heterotrimeric guanine nucleotide-binding protein 1C
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04899
  • Protein Name
    Guanine nucleotide-binding protein G(i) subunit alpha-2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of GNAI2 using anti-GNAI2 antibody (A02498-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNAI2 antigen affinity purified polyclonal antibody (Catalog # A02498-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GNAI2 at approximately 40 kDa. The expected band size for GNAI2 is at 40 kDa.
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