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GNAI2 Monoclonal Antibody

BSM-60516M
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGNAI2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    GNAI2 Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(), ICC/IF(1:50-100)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    B3E3
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2771
  • Target name
    GNAI2
  • Target description
    G protein subunit alpha i2
  • Target synonyms
    adenylate cyclase-inhibiting G alpha protein; GIP; GNAI2B; GTP-binding regulatory protein Gi alpha-2 chain; guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2; guanine nucleotide-binding protein G(i) subunit alpha-2; guanine nucleotide-binding protein G(i), alpha-2 subunit; H_LUCA15.1; H_LUCA16.1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP04899
  • Protein Name
    Guanine nucleotide-binding protein G(i) subunit alpha-2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GNAI2 using anti-GNAI2 antibody (A02498-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNAI2 antigen affinity purified polyclonal antibody (Catalog # A02498-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GNAI2 at approximately 40 kDa. The expected band size for GNAI2 is at 40 kDa.
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