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Immunohistochemistry analysis in human cerebral cortex and pancreas tissues using Anti-GNAO1 antibody. Corresponding GNAO1 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human cerebral cortex and pancreas tissues using Anti-GNAO1 antibody. Corresponding GNAO1 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human cerebral cortex and pancreas tissues using Anti-GNAO1 antibody. Corresponding GNAO1 RNA-seq data are presented for the same tissues.

Anti-GNAO1 Antibody

HPA040878
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGNAO1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GNAO1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2775
  • Target name
    GNAO1
  • Target description
    G protein subunit alpha o1
  • Target synonyms
    DEE17, EIEE17, G-ALPHA-o, GNAO, HG1G, HLA-DQB1, NEDIM, guanine nucleotide-binding protein G(o) subunit alpha, GO2-q chimeric G-protein, guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O, guanine nucleotide-binding regulatory protein 2, heterotrimeric guanine nucleotide-binding protein 1G
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09471
  • Protein Name
    Guanine nucleotide-binding protein G(o) subunit alpha
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of GNAO1 using anti-GNAO1 antibody (A05532-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNAO1 antigen affinity purified polyclonal antibody (Catalog # A05532-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GNAO1 at approximately 40 kDa. The expected band size for GNAO1 is at 40 kDa.
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