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Immunohistochemical staining of human colon shows distinct positivity in endothelial cells.
Immunohistochemical staining of human colon shows distinct positivity in endothelial cells.
Immunohistochemical staining of human colon shows distinct positivity in endothelial cells.

Anti-GNG2 Antibody

HPA003534
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGNG2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GNG2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54331
  • Target name
    GNG2
  • Target description
    G protein subunit gamma 2
  • Target synonyms
    HG3F1, guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, g gamma-I, guanine nucleotide binding protein (G protein), gamma 2, guanine nucleotide binding protein gamma 2, guanine nucleotide-binding protein G(I)/G(O) gamma-2 subunit, heterotrimeric guanine nucleotide-binding protein 3F1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP59768
  • Protein Name
    Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of GNG2 using anti-GNG2 antibody (A06975-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U87 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human HL-60 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat C6 whole cell lysates, Lane 9: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNG2 antigen affinity purified polyclonal antibody (Catalog # A06975-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GNG2 at approximately 12KD. The expected band size for GNG2 is at 12KD.
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