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WB analysis of various sample lysates using GTX64993 GNG2 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX64993 GNG2 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX64993 GNG2 antibody. Dilution : 1:1000 Loading : 25microg per lane

GNG2 antibody

GTX64993
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetGNG2
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Overview

  • Supplier
    GeneTex
  • Product Name
    GNG2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54331
  • Target name
    GNG2
  • Target description
    G protein subunit gamma 2
  • Target synonyms
    HG3F1, guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, g gamma-I, guanine nucleotide binding protein (G protein), gamma 2, guanine nucleotide binding protein gamma 2, guanine nucleotide-binding protein G(I)/G(O) gamma-2 subunit, heterotrimeric guanine nucleotide-binding protein 3F1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP59768
  • Protein Name
    Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
  • Scientific Description
    This gene encodes one of the gamma subunits of a guanine nucleotide-binding protein. Such proteins are involved in signaling mechanisms across membranes. Various subunits forms heterodimers which then interact with the different signal molecules. [provided by RefSeq, Aug 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GNG2 using anti-GNG2 antibody (A06975-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U87 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human HL-60 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat C6 whole cell lysates, Lane 9: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNG2 antigen affinity purified polyclonal antibody (Catalog # A06975-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GNG2 at approximately 12KD. The expected band size for GNG2 is at 12KD.
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