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Immunohistochemical staining of human caudate shows strong positivity in neuronal projections.
Immunohistochemical staining of human caudate shows strong positivity in neuronal projections.
Immunohistochemical staining of human caudate shows strong positivity in neuronal projections.

Anti-GNG7 Antibody

HPA057790
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGNG7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GNG7 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2788
  • Target name
    GNG7
  • Target description
    G protein subunit gamma 7
  • Target synonyms
    HG3B, guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-7, guanine nucleotide binding protein (G protein), gamma 7, heterotrimeric guanine nucleotide-binding protein 3B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60262
  • Protein Name
    Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Immunofluorescence staining of MCF-7 cells with CSB-PA009619LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Figure 1. Western blot analysis of GNG7 using anti-GNG7 antibody (A10690-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U937 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNG7 antigen affinity purified polyclonal antibody (Catalog # A10690-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GNG7 at approximately 12KD. The expected band size for GNG7 is at 12KD.
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