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GNG7 Antibody

LS-C496718
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGNG7
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GNG7 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 7kDa, while the observed MW by Western blot was 12kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:1000) The predicted MW is 7kDa, while the observed MW by Western blot was 12kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2788
  • Target name
    GNG7
  • Target description
    G protein subunit gamma 7
  • Target synonyms
    HG3B, guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-7, guanine nucleotide binding protein (G protein), gamma 7, heterotrimeric guanine nucleotide-binding protein 3B
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GNG7 using anti-GNG7 antibody (A10690-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U937 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNG7 antigen affinity purified polyclonal antibody (Catalog # A10690-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GNG7 at approximately 12KD. The expected band size for GNG7 is at 12KD.
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