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Immunohistochemical staining of human endometrium, gastrointestinal, kidney and prostate using Anti-GOLGB1 antibody HPA011555 (A) shows similar protein distribution across tissues to independent antibody HPA011008 (B).
Immunohistochemical staining of human endometrium, gastrointestinal, kidney and prostate using Anti-GOLGB1 antibody HPA011555 (A) shows similar protein distribution across tissues to independent antibody HPA011008 (B).
Immunohistochemical staining of human endometrium, gastrointestinal, kidney and prostate using Anti-GOLGB1 antibody HPA011555 (A) shows similar protein distribution across tissues to independent antibody HPA011008 (B).

Anti-GOLGB1 Antibody

HPA011555
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetGOLGB1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GOLGB1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2804
  • Target name
    GOLGB1
  • Target description
    golgin B1
  • Target synonyms
    372 kDa Golgi complex-associated protein; GCP; GCP372; giantin; golgi autoantigen, golgin subfamily b, macrogolgin (with transmembrane signal), 1; golgi integral membrane protein 1; golgin B1, golgi integral membrane protein; golgin subfamily B member 1; GOLIM1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14789
  • Protein Name
    Golgin subfamily B member 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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IHC image of CSB-PA622788LA01HU diluted at 1:300 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of Giantin/GOLGB1 using anti-Giantin/GOLGB1 antibody (A08226-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Giantin/GOLGB1 antigen affinity purified polyclonal antibody (Catalog # A08226-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Giantin/GOLGB1 at approximately 376 kDa. The expected band size for Giantin/GOLGB1 is at 376 kDa.
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Immunohistochemical staining of human endometrium, gastrointestinal, kidney and prostate using Anti-GOLGB1 antibody HPA011008 (A) shows similar protein distribution across tissues to independent antibody HPA011555 (B).
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Hela cells probed with Giantin/GM130 Polyclonal Antibody, Unconjugated (bs-13356R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
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