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Immunofluorescent staining of human cell line Hep G2 shows localization to plasma membrane.
Immunofluorescent staining of human cell line Hep G2 shows localization to plasma membrane.
Immunofluorescent staining of human cell line Hep G2 shows localization to plasma membrane.

Anti-GPC3 Antibody

HPA006316
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetGPC3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GPC3 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2719
  • Target name
    GPC3
  • Target description
    glypican 3
  • Target synonyms
    DGSX, GTR2-2, MXR7, OCI-5, SDYS, SGB, SGBS, SGBS1, glypican-3, glypican proteoglycan 3, heparan sulphate proteoglycan, intestinal protein OCI-5, secreted glypican-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51654
  • Protein Name
    Glypican-3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of Glypican 3/GPC3 using anti-Glypican 3/GPC3 antibody (A01922-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glypican 3/GPC3 antigen affinity purified polyclonal antibody (Catalog # A01922-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glypican 3/GPC3 at approximately 66 kDa. The expected band size for Glypican 3/GPC3 is at 66 kDa.
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