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Western blot analysis using anti-GPX1 (human), pAb (Prod. No. AG-25A-0104) at 1:2000 dilution. 1: Human GPX1 (His-tagged). 2: Human GPX2 (His-tagged). 3: Human GPX3 (His-tagged).
Western blot analysis using anti-GPX1 (human), pAb (Prod. No. AG-25A-0104) at 1:2000 dilution. 1: Human GPX1 (His-tagged). 2: Human GPX2 (His-tagged). 3: Human GPX3 (His-tagged).
Western blot analysis using anti-GPX1 (human), pAb (Prod. No. AG-25A-0104) at 1:2000 dilution. 1: Human GPX1 (His-tagged). 2: Human GPX2 (His-tagged). 3: Human GPX3 (His-tagged).

anti-GPX1 (human), pAb

AG-25A-0104
AdipoGen Life Sciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetGPX1
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Overview

  • Supplier
    AdipoGen Life Sciences
  • Product Name
    anti-GPX1 (human), pAb
  • Delivery Days Customer
    10
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID2876
  • Target name
    GPX1
  • Target description
    glutathione peroxidase 1
  • Target synonyms
    cellular glutathione peroxidase; glutathione peroxidase 1; GPXD; GSHPX1; GSHPx-1; selenoprotein GPX1
  • Host
    Rabbit
  • Protein IDP07203
  • Protein Name
    Glutathione peroxidase 1
  • Scientific Description
    GPX1 is an antioxidant enzyme that is down-regulated in cancer cells. Protects the hemoglobin in erythrocytes from oxidative breakdown. Its main role is the prevention of cancer initiation by ROS-mediated DNA damage. - Polyclonal Antibody. Recognizes human GPX1. Detects a band of ~23kDa by Western blot. Source: Rabbit. Applications: ELISA, WB. Liquid. 0.2microm-filtered solution in PBS, pH 7.4. Contains no preservatives. GPX1 is an antioxidant enzyme that is down-regulated in cancer cells. Protects the hemoglobin in erythrocytes from oxidative breakdown. Its main role is the prevention of cancer initiation by ROS-mediated DNA damage.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GPX1 using anti-GPX1 antibody (A01019-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPX1 antigen affinity purified polyclonal antibody (Catalog # A01019-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPX1 at approximately 22 kDa. The expected band size for GPX1 is at 22 kDa.
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