IHC analysis of GZMB using anti-GZMB antibody (M00353-1) on paraffin-embedded human T cell lymphoma. GZMB was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GZMB Antibody (M00353-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of GZMB using anti-GZMB antibody (M00353-1) on paraffin-embedded human T cell lymphoma. GZMB was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GZMB Antibody (M00353-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Granzyme B GZMB Monoclonal Antibody
M00353-1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGZMB
Overview
- SupplierBoster Bio
- Product NameAnti-Granzyme B Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDAAHC-7
- FormulationLiquid
- Gene ID3002
- Target nameGZMB
- Target descriptiongranzyme B
- Target synonymsC11; cathepsin G-like 1; CCPI; CGL1; CGL-1; CSPB; CSP-B; CTLA1; CTSGL1; cytotoxic serine protease B; cytotoxic T-lymphocyte proteinase 2; fragmentin 2; granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); HLP; human lymphocyte protein; SECT; T-cell serine protease 1-3E
- HostRabbit
- IsotypeIgG
- Protein IDP10144
- Protein NameGranzyme B
- Scientific DescriptionBoster Bio Anti-Granzyme B GZMB Monoclonal Antibody catalog # M00353-1. Tested in IHC, ICC/IF, IP applications. This antibody reacts with Human.
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203