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Anti-GRB14 Antibody

144-65581
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetGRB14
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-GRB14 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID2888
  • Target name
    GRB14
  • Target description
    growth factor receptor bound protein 14
  • Target synonyms
    growth factor receptor-bound protein 14, GRB14 adapter protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14449
  • Protein Name
    Growth factor receptor-bound protein 14
  • Scientific Description
    GRB14 pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GRB14 using anti-GRB14 antibody (A04177-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRB14 antigen affinity purified polyclonal antibody (Catalog # A04177-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRB14 at approximately 61KD. The expected band size for GRB14 is at 61KD.
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