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Western Blot analysis of MCF7 cells using GRB14 Polyclonal Antibody
Western Blot analysis of MCF7 cells using GRB14 Polyclonal Antibody
Western Blot analysis of MCF7 cells using GRB14 Polyclonal Antibody

GRB14 Antibody

CSB-PA002809
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGRB14
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Overview

  • Supplier
    Cusabio
  • Product Name
    GRB14 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2888
  • Target name
    GRB14
  • Target description
    growth factor receptor bound protein 14
  • Target synonyms
    growth factor receptor-bound protein 14, GRB14 adapter protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14449
  • Protein Name
    Growth factor receptor-bound protein 14
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GRB14 using anti-GRB14 antibody (A04177-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRB14 antigen affinity purified polyclonal antibody (Catalog # A04177-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRB14 at approximately 61KD. The expected band size for GRB14 is at 61KD.
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