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Anti-GREM1 Antibody

144-11595
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetGREM1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-GREM1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID26585
  • Target name
    GREM1
  • Target description
    gremlin 1, DAN family BMP antagonist
  • Target synonyms
    C15DUPq; cell proliferation-inducing gene 2 protein; CKTSF1B1; colorectal adenoma and carcinoma 1; CRAC1; CRCS4; cysteine knot superfamily 1, BMP antagonist 1; DAN domain family member 2; DAND2; down-regulated in Mos-transformed cells protein; DRM; DUP15q; GREMLIN; gremlin 1, cysteine knot superfamily, homolog; gremlin 1-like protein; gremlin-1; hereditary mixed polyposis syndrome; HMPS; HMPS1; IHG-2; increased in high glucose-2; MPSH; PIG2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60565
  • Protein Name
    Gremlin-1
  • Scientific Description
    GREM1 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GREM1 using anti-GREM1 antibody (PB9626). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GREM1 antigen affinity purified polyclonal antibody (Catalog # PB9626) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GREM1 at approximately 21 kDa. The expected band size for GREM1 is at 21 kDa.
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