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Immunohistochemistry analysis in human prostate and liver tissues using AMAb91226 antibody. Corresponding GRHL2 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human prostate and liver tissues using AMAb91226 antibody. Corresponding GRHL2 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human prostate and liver tissues using AMAb91226 antibody. Corresponding GRHL2 RNA-seq data are presented for the same tissues.

Anti-GRHL2 Antibody

AMAB91226
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGRHL2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GRHL2
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL3760
  • Conjugate
    Unconjugated
  • Gene ID79977
  • Target name
    GRHL2
  • Target description
    grainyhead like transcription factor 2
  • Target synonyms
    BOM, DFNA28, ECTDS, PPCD4, TFCP2L3, grainyhead-like protein 2 homolog, brother of mammalian grainyhead, brother-of-MGR, grainyhead-like 2, transcription factor CP2-like 3
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ6ISB3
  • Protein Name
    Grainyhead-like protein 2 homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GRHL2 using anti-GRHL2 antibody (A04120-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRHL2 antigen affinity purified polyclonal antibody (Catalog # A04120-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRHL2 at approximately 71 kDa. The expected band size for GRHL2 is at 71 kDa.
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