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Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.

Anti-GRHL2 Antibody

HPA004820
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGRHL2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GRHL2
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79977
  • Target name
    GRHL2
  • Target description
    grainyhead like transcription factor 2
  • Target synonyms
    BOM, DFNA28, ECTDS, PPCD4, TFCP2L3, grainyhead-like protein 2 homolog, brother of mammalian grainyhead, brother-of-MGR, grainyhead-like 2, transcription factor CP2-like 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6ISB3
  • Protein Name
    Grainyhead-like protein 2 homolog
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GRHL2 using anti-GRHL2 antibody (A04120-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRHL2 antigen affinity purified polyclonal antibody (Catalog # A04120-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRHL2 at approximately 71 kDa. The expected band size for GRHL2 is at 71 kDa.
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