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Anti-GRHL2 Antibody

ORB2596710
Biorbyt
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetGRHL2
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Anti-GRHL2 Antibody
  • Delivery Days Customer
    10
  • Application Supplier Note
    Western blot, 0.25-0.5 microg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 microg/ml, Human Immunocytochemistry/Immunofluorescence, 5 microg/ml, Human Flow Cytometry (Fixed), 1-3 microg/1x106 cells, Human ELISA, 0.1-0.5 microg/ml, -. Adding 0.2 ml of distilled water will yield a concentration of 500 microg/ml
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, FC, ICC, IF, IHC, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Cy3
  • Gene ID79977
  • Target name
    GRHL2
  • Target description
    grainyhead like transcription factor 2
  • Target synonyms
    BOM, DFNA28, ECTDS, PPCD4, TFCP2L3, grainyhead-like protein 2 homolog, brother of mammalian grainyhead, brother-of-MGR, grainyhead-like 2, transcription factor CP2-like 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6ISB3
  • Protein Name
    Grainyhead-like protein 2 homolog
  • Scientific Description
    Anti-GRHL2 Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GRHL2 using anti-GRHL2 antibody (A04120-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRHL2 antigen affinity purified polyclonal antibody (Catalog # A04120-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRHL2 at approximately 71 kDa. The expected band size for GRHL2 is at 71 kDa.
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