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Immunohistochemical staining of human hippocampus shows strong positivity in neuronal processes in neuropil.
Immunohistochemical staining of human hippocampus shows strong positivity in neuronal processes in neuropil.
Immunohistochemical staining of human hippocampus shows strong positivity in neuronal processes in neuropil.

Anti-GRIA1 Antibody

HPA035202
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGRIA1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GRIA1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2890
  • Target name
    GRIA1
  • Target description
    glutamate ionotropic receptor AMPA type subunit 1
  • Target synonyms
    GLUH1, GLUR1, GLURA, GluA1, HBGR1, MRD67, MRT76, glutamate receptor 1, AMPA 1, AMPA receptor subunit GluA1, AMPA-selective glutamate receptor 1, gluR-1, gluR-A, gluR-K1, glutamate receptor, ionotropic, AMPA 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42261
  • Protein Name
    Glutamate receptor 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of GRIA1 using anti-GRIA1 antibody (PB9204). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: Recombinant Human GRIA1 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIA1 antigen affinity purified polyclonal antibody (Catalog # PB9204) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRIA1 at approximately 40KD. The expected band size for GRIA1 is at 40KD.
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