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Western Blot analysis of COLO205 cells using GluR-1 Polyclonal Antibody
Western Blot analysis of COLO205 cells using GluR-1 Polyclonal Antibody
Western Blot analysis of COLO205 cells using GluR-1 Polyclonal Antibody

GRIA1 Antibody

CSB-PA002723
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGRIA1
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Overview

  • Supplier
    Cusabio
  • Product Name
    GRIA1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2890
  • Target name
    GRIA1
  • Target description
    glutamate ionotropic receptor AMPA type subunit 1
  • Target synonyms
    GLUH1, GLUR1, GLURA, GluA1, HBGR1, MRD67, MRT76, glutamate receptor 1, AMPA 1, AMPA receptor subunit GluA1, AMPA-selective glutamate receptor 1, gluR-1, gluR-A, gluR-K1, glutamate receptor, ionotropic, AMPA 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42261
  • Protein Name
    Glutamate receptor 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GRIA1 using anti-GRIA1 antibody (PB9204). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: Recombinant Human GRIA1 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIA1 antigen affinity purified polyclonal antibody (Catalog # PB9204) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRIA1 at approximately 40KD. The expected band size for GRIA1 is at 40KD.
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