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Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in molecular layer.
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in molecular layer.
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in molecular layer.

Anti-GRID2 Antibody

HPA056253
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetGRID2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GRID2
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2895
  • Target name
    GRID2
  • Target description
    glutamate ionotropic receptor delta type subunit 2
  • Target synonyms
    GluD2; gluR delta-2 subunit; glutamate receptor delta-2 subunit; glutamate receptor ionotropic, delta-2; glutamate receptor, ionotropic, delta 2; SCAR18
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43424
  • Protein Name
    Glutamate receptor ionotropic, delta-2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GRID2 using anti-GRID2 antibody (A00410-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRID2 antigen affinity purified polyclonal antibody (Catalog # A00410-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRID2 at approximately 113KD. The expected band size for GRID2 is at 113KD.
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