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Western Blot analysis of 3T3 cells using GluR-delta2 Polyclonal Antibody
Western Blot analysis of 3T3 cells using GluR-delta2 Polyclonal Antibody
Western Blot analysis of 3T3 cells using GluR-delta2 Polyclonal Antibody

GRID2 Antibody

CSB-PA008961
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGRID2
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Overview

  • Supplier
    Cusabio
  • Product Name
    GRID2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2895
  • Target name
    GRID2
  • Target description
    glutamate ionotropic receptor delta type subunit 2
  • Target synonyms
    GluD2, SCAR18, glutamate receptor ionotropic, delta-2, gluR delta-2 subunit, glutamate receptor delta-2 subunit, glutamate receptor, ionotropic, delta 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43424
  • Protein Name
    Glutamate receptor ionotropic, delta-2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GRID2 using anti-GRID2 antibody (A00410-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRID2 antigen affinity purified polyclonal antibody (Catalog # A00410-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRID2 at approximately 113KD. The expected band size for GRID2 is at 113KD.
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