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Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane.
Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane.
Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane.

Anti-GRIK1 Antibody

HPA073879
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetGRIK1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GRIK1 Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2897
  • Target name
    GRIK1
  • Target description
    glutamate ionotropic receptor kainate type subunit 1
  • Target synonyms
    EAA3, EEA3, GLR5, GLUR5, GluK1, gluR-5, glutamate receptor ionotropic, kainate 1, GluK1(del_e2), excitatory amino acid receptor 3, glutamate receptor 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP39086
  • Protein Name
    Glutamate receptor ionotropic, kainate 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GRIK1 using anti-GRIK1 antibody (PB9208). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human GRIK1 protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIK1 antigen affinity purified polyclonal antibody (Catalog # PB9208) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRIK1 at approximately 40 kDa. The expected band size for GRIK1 is at 40 kDa.
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